Figure 2. The effect on refractive compensation of the loop diuretic bumetanide at two concentrations in the vitreous chamber compared with control saline injections.

Bum1 refers to an effective vitreal concentration of 10⁻⁵ M, Bum2 refers to an effective concentration of 5×10⁻⁶ M. A. Scatter plot of Refraction in dioptres versus Vitreous Chamber depth (mm) across all eyes measured. The applied defocus is indicated by the triangles or circle symbols, with red filled symbols indicating Bum1, red open symbols indicating Bum2 and blue symbols indicating saline injected eyes. B. Compensation to negative lens rearing markedly diminished for both bumetanide doses with the higher dose (Bum1) resulting in approximately 6D less refractive compensation than for SAL. The same colour code applies as for A. The refractive effect is specific to the induction of myopia, with 0D and +10D groups showing no significant effect of the intravitreal diuretic. C. Vitreous chamber depths of bumetanide and saline injected eyes. The same colour code applies as for A. While the same trend as in A is evident with a dose dependency with negative lens defocus, it can also be seen that mean VC depth is slightly larger for Bumetanide cf SAL eyes, especially for 0D and +10D groups. Data presented as means±SE.