Figure 3. Inhibition of death receptor-induced apoptosis correlates with enhanced clonogenic survival in HeLa Bid kd cells.

Control cells and HeLa Bid kd cells were treated with CHX (1 µg/ml) in combination with an agonistic FAS antibody or recombinant TRAIL for the indicated times. Controls were treated with vehicle. After incubation, 1000 cells were transferred to 60 mm dishes and cultured with fresh medium for 14 days. Then colonies were fixed, stained with methylene blue and counted. A, B) Colony formation in representative dishes is shown. C, D) Graphical representation of the percentage of colonies after treatment compared to control cells treated with vehicle (100%). Data are means+/−SD from at least two independent experiments performed in triplicate. # p<0.05: difference from control cells (Ctrl). ∗ p<0.05: difference from Bid kd cells treated with vehicle.