Figure 1.

Coomassie-stained two-dimensional separation of L. pulchella Tnt isoforms (arrows; identity was confirmed by Western blots of the same gels). (Upper) An individual with two predominant protein isoforms; the same individual showed two predominant mRNA fragment sizes (Inset from genescan analysis). (Lower) An individual with four or perhaps five predominant protein isoforms; this individual showed four predominant mRNA fragment sizes. Bars at top show pH 5.3 (Left) and 5.75 (Right). Bars on the vertical scale show molecular masses of 94 kDa (Upper) and 60 kDa (Lower). TnT isoforms had an apparent molecular mass of 44.3 kDa on these gels. Numbers on genescan insets are defined in Table 1.