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. 2008 Jun 17;10(3):R53. doi: 10.1186/bcr2108

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Copyright © 2008 Honeth et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemical double-staining of human breast tumors for CD44 and CD24. CD44 is stained with Permanent Red and CD24 with diaminobenzidene (DAB). Magnification × 20. (a) A tumor positive for both CD44⁺/CD24^-(white arrow) and CD44^-/CD24⁺(black arrow) cancer cells, although the predominant phenotype is CD44^-/CD24^-. (b) Almost all cells in this tumor are CD44⁺/CD24^-. No CD24 staining is seen. (c) A tumor with predominantly CD44⁺/CD24^-cells. A few CD44⁺/CD24⁺cells are also present (black arrow). (d) A tumor positive for the CD44^-/CD24⁺phenotype. No CD44 staining is present.