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Electrophoresis on agarose gel of VLPs found in crude lysates of cells producing a coat proteins with different random 6-, 8- or 10-amino acid insertions. The first two lanes of each gel are controls: pUCter3 produces no coat protein, while p2MCTK3 is the single-chain dimer construct without a peptide insertion. Ethidium bromide-stained gels (upper half of each set) and blots probed with anti-MS2 serum (lower half of each set) are shown.
