Table 3.

DNA oligonucleotides used in this study

Oligonucleotide	5′-Sequenz-3′
AMPLI1	TAGTCAAGCTTCGCCATTACAAAGAGC
AMPLI3	CTACTAAGCTTTTCCACTGGTGCATC
AMPLI6	CTACTACTAGTGTCGACGTTTTCCACTGGTGCATC
DTNOT	AATTCGCGGCCGCTTTTTTTTTTTTTTTT
ECOMUPL3	TAGTCGAATTCTTGCTGAGATAG
NOTTAG	TAATTGCGGCCGCTGCAGNWNNSNCTCAGTAACAGATACTTG
P3_1340	TATCGTGCCATGCTTAATGG
P3_2305	CCAATCCCGCCATCTATAAC
PLB1390	GACTGCTCGAGTGTAGAACTTGCTGAATCG
RACE3	CAGATGTAAGTGACGAGG
UPPLIP3	TCCACCACTGAATGACAACC
XNTAG	CAGGGACGACTGCTCGAGNRNNRNTGTAGAACTTGCTGAATCG
PLB1-A	GATGAATGGGCAGCATGTGTT
PLB1-B	GGCTCACCCTTATAGATGGTACCA
PLB1-D	GCATCTCTTACATTGTTCTGTCTGTTC
PLB1-E (Probe)	TCTTTCTTGCTCTCTCCGTATGATGGCG
PLB2-A	AGGTGAAGACGGTCAGAATGTTC
PLB2-B	GCTGATCCATCTGGCCAATT
PLB2-C	ATGTTCCCTTGCTTCCATTGA
PLB2-D	TGTTTTTATCTGCCGATTGATCA
PLB2-E (Probe)	CCACCGTAAGGTAAGCGCAATCTTTGC
PLB3-A	AAGAAAGACGTGGTATTGAACAACTG
PLB3-B	TGCTTCCATTAGTTAAACTTGAATCAG
PLB3-C	TGGTATTGAACAACTGGATCAATGT
PLB3-E (Probe)	AAGTACCATCCCAACAATAATT
PLB4-A	TCGCTTATGATAATTCGGCTGATA
PLB4-B	CCACATCAGGTACATATGGGAAACT
PLB4-C	TGCCTCCATGGTAGCATCATAC
PLB4-E (Probe)	ATTTGGGAATCAAAGTAATGG
PLB5-A	CGCTGCCTCTGTCTCTAGTGTTAG
PLB5-B	GATGATCCGGTAGTGGTACTGGTT
PLB5-C	CTTGCAGCTAAAACCCACACAA
PLB5-D	GATCCGGTAGTGGTACTGGTTTG
PLB5-E (Probe)	TGGCGGTACATCTTCCACGACCCA

Restriction enzyme sites are underlined and sequence tags are in bold. Oligonucleotides for nested and semi-nested real-time PCR are italicized.