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. 2008 Apr 8;112(3):592–602. doi: 10.1182/blood-2007-09-110437

Figure 4.

Figure 4

Effect of myr-RGT on soluble fibrinogen binding to platelets. Platelets were preincubated with different peptides or their vehicle, and binding of Alexa Fluor 488-conjugated fibrinogen (100 μg/mL) to platelets was measured by flow cytometry after the addition of 20 μM of ADP. (A) Representative histograms. Fibrinogen bound to platelets treated with DMSO, myristic acid, and RGT at 250 μM (M + RGT), scrambled myr-GRT at 250 μM (myr-GRT), myr-RGT at 250 μM (myr-RGT), or with RGDS peptide (1 mM). The background fibrinogen binding was assessed using platelets treated without ADP (Control). (B) Statistical data were derived from quantitative results (means and SD) calculated from the ratios of mean fluorescence intensity (samples/control) of 3 separate experiments.