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. 2008 Aug;10(8):773–781. doi: 10.1593/neo.08462

Figure 5.

Figure 5

HMGA1 knock down impairs HPV E6/E7 transcription. (A) HeLa cells transfected with the si-A1-pool and the si-NT as described in the Materials and Methods section were then retransfected with the PGL3 vector (black bars) or the pGL3-HPV18LCR reporter construct (white bars) and analyzed for luciferase activity. (B) HeLa cells transfected with the si-A1-pool and the si-NT as described in the Materials and Methods section were then analyzed by quantitative PCR for the expression of HMGA1 (black bars) or HPV18 E6/E7 (white bars) transcripts normalized on GAPDH.