Figure 1.

Increased stability of the FGFR-4 Arg³⁸⁸ variant after ligand stimulation. 293T cells were transfected with V5-tagged FGFR-4 Arg³⁸⁸ or Gly³⁸⁸ and cell surface receptors labeled with biotin. Cells were then stimulated with FGF2 and lysed at the indicated time. Labeled receptors were then immunoprecipitated with streptavidin-agarose and FGFR-4 detected by Western blot of the immunoprecipitates with anti-V5 antibody. Western blot of an aliquot of the lysate used for immunoprecipitation with anti-β-actin antibody is shown.