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. 1970 Oct;104(1):401–409. doi: 10.1128/jb.104.1.401-409.1970

Cytological Studies of Deoxyribonucleic Acid Replication in Escherichia coli 15T−: Replication at Slow Growth Rates and After a Shift-Up into Rich Medium

Ning-Chun Chai 1, K G Lark 1
PMCID: PMC248226  PMID: 4919753

Abstract

We examined the gross nuclear morphology of Escherichia coli 15T− grown in different media with doubling times ranging from 22 to 270 min. In slowly growing cells, deoxyribonucleic acid synthesis was measured by autoradiography and shown to occur with greatest probability during the first two-thirds of the division cycle. In such cells, segregation occurred later, at the end of the division cycle rather than at the end of deoxyribonucleic acid replication. Nuclear regions in L-broth cells (22-min doubling time) cannot correspond to separate chromosomes but probably represent regions of replication activity. Segregation of template nucleotide strands was measured after a shift-up from proline M9 or glucose M9 media into L broth. A model is presented to account for the pattern of segregation observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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