Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Oct;104(1):401–409. doi: 10.1128/jb.104.1.401-409.1970

Cytological Studies of Deoxyribonucleic Acid Replication in Escherichia coli 15T−: Replication at Slow Growth Rates and After a Shift-Up into Rich Medium

Ning-Chun Chai 1, K G Lark 1
PMCID: PMC248226  PMID: 4919753

Abstract

We examined the gross nuclear morphology of Escherichia coli 15T− grown in different media with doubling times ranging from 22 to 270 min. In slowly growing cells, deoxyribonucleic acid synthesis was measured by autoradiography and shown to occur with greatest probability during the first two-thirds of the division cycle. In such cells, segregation occurred later, at the end of the division cycle rather than at the end of deoxyribonucleic acid replication. Nuclear regions in L-broth cells (22-min doubling time) cannot correspond to separate chromosomes but probably represent regions of replication activity. Segregation of template nucleotide strands was measured after a shift-up from proline M9 or glucose M9 media into L broth. A model is presented to account for the pattern of segregation observed.

Full text

PDF
403

Images in this article

403Image
on p.403
404Image
on p.404
405Image
on p.405
406Image
on p.406

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bird R. E., Lark K. G. Chromosome replication in Escherichia coli 15T- at different growth rates: rate of replication of the chromosome and the rate of formation of small pieces. J Mol Biol. 1970 Apr 28;49(2):343–366. doi: 10.1016/0022-2836(70)90249-4. [DOI] [PubMed] [Google Scholar]
  2. Bird R., Lark K. G. Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-. Cold Spring Harb Symp Quant Biol. 1968;33:799–808. doi: 10.1101/sqb.1968.033.01.092. [DOI] [PubMed] [Google Scholar]
  3. Chai N. C., Lark K. G. Segregation of deoxyribonucleic acid in bacteria: association of the segregating unit with the cell envelope. J Bacteriol. 1967 Aug;94(2):415–421. doi: 10.1128/jb.94.2.415-421.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  5. Eberle H., Lark K. G. Chromosome replication in Bacillus subtilis cultures growing at different rates. Proc Natl Acad Sci U S A. 1967 Jan;57(1):95–101. doi: 10.1073/pnas.57.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helmstetter C. E. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. doi: 10.1016/0022-2836(68)90424-5. [DOI] [PubMed] [Google Scholar]
  7. Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
  8. KELLENBERGER E., LARK K. G., BOLLE A. Amino acid dependent control of DNA synthesis in bacteria and vegetative phage. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1860–1868. doi: 10.1073/pnas.48.10.1860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lark C. Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates. Biochim Biophys Acta. 1966 Jun 22;119(3):517–525. doi: 10.1016/0005-2787(66)90128-6. [DOI] [PubMed] [Google Scholar]
  10. Lark K. G., Bird R. E. Segregation of the conserved units of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1965 Nov;54(5):1444–1450. doi: 10.1073/pnas.54.5.1444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. POWELL E. O. Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol. 1956 Dec;15(3):492–511. doi: 10.1099/00221287-15-3-492. [DOI] [PubMed] [Google Scholar]
  13. RYTER A., KELLENBERGER E. L'inclusion au polyester pour l'ultramicrotomie. J Ultrastruct Res. 1958 Dec;2(2):200–214. doi: 10.1016/s0022-5320(58)90018-2. [DOI] [PubMed] [Google Scholar]
  14. Ryter A. Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriol Rev. 1968 Mar;32(1):39–54. doi: 10.1128/br.32.1.39-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ryter A., Hirota Y., Jacob F. DNA-membrane complex and nuclear segregation in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:669–676. doi: 10.1101/sqb.1968.033.01.076. [DOI] [PubMed] [Google Scholar]
  16. SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
  17. SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]
  18. Sueoka N., Quinn W. G. Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968;33:695–705. doi: 10.1101/sqb.1968.033.01.078. [DOI] [PubMed] [Google Scholar]
  19. YOSHIKAWA H., O'SULLIVAN A., SUEOKA N. SEQUENTIAL REPLICATION OF THE BACILLUS SUBTILIS CHROMOSOME. 3. REGULATION OF INITIATION. Proc Natl Acad Sci U S A. 1964 Oct;52:973–980. doi: 10.1073/pnas.52.4.973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES