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. 1999 Dec 21;96(26):15354–15361. doi: 10.1073/pnas.96.26.15354

Figure 4.

Figure 4

Localization of PvNCED1. (A) Import of PvNCED1 into pea chloroplasts. Lanes: 1, in vitro translated nascent PvNCED1; 2–5, pea chloroplasts incubated with PvNCED1 for 15 s, 5, 10, and 20 min, respectively, then treated with thermolysin. The chloroplasts were reisolated and analyzed in 9% SDS/PAGE; 6 and 7, soluble and membrane fractions, respectively, of chloroplasts that had been incubated with PvNCED1 for 20 min. (B) Immunoblot of protein extracted from detached bean leaves that were either kept turgid (0 h), or were dehydrated for 4 or 24 h. Cytosolic (Cyt), chloroplast (Chlp), and thylakoid (Thy) fractions were separated for each leaf sample. Each lane was loaded with 30 μg of protein. The membranes were probed with antiserum raised against recombinant PvNCED1.