Figure 8.

Effect of dehydration for 4 h and rehydration during the subsequent 4 h on PvNCED1 transcript and protein levels, and ABA content in roots. (A) Time course of changes in PvNCED1 mRNA, PvNCED1 protein, and ABA levels in detached bean roots in response to dehydration and rehydration. (B) Protein gel blot cross-reacted with antiserum raised against recombinant PvNCED1 (Top), and RNA gel blot analysis of the expression of PvNCED1 (Middle), or Actin (Bottom) as loading control. The ratio of PvNCED1/Actin mRNA is plotted in A as relative intensity. Dh, dehydrated; Rh, rehydrated.