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The effect of low temperature on stress-induced ABA and expression of PvNCED1. (A) ABA levels and relative intensity of PvNCED1 mRNA of nonstressed (Ns) and dehydrated leaves (S) kept at different temperatures. (B) RNA gel blot analysis of PvNCED1 mRNA (Upper) or Actin as control. The ratio of PvNCED1/Actin mRNA is plotted in A as relative intensity. Dehydrated leaves (S) were kept at 22, 7, or 2°C for 5 or 24 h. Nonstressed (Ns) leaves were kept at the same temperatures for 24 h.
