Figure 1.

(A) Internal peptide sequences of the purified C. album Chlase (type 0). The amino acid expressed as “X” and underlined in AP-2 is the putative modifiable amino acid (see text). (B) Nucleotide and deduced amino acid sequences of CaCLH. The internal peptide sequences shown in A are highlighted. The putative signal sequence for ER is underlined, and the putative cleavage site for mature protein is indicated by the arrowhead. The dashed underline indicates the sequence identical to NPIR central motif, a vacuolar-sorting determinant conserved in prosporamin and proaleurain (26).