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Correlation between cefotaxime acylation efficiency and hydrolytic activity using the thiolester substrate, S2d. Best fit: (k2/K) = −6,630.0 + 189.0 (kcat/Km), r = 0.94. RC, clinical resistant Sp328 (R-PBP2x*); RC-A338T, R-PBP2x* A338T mutant.
