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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Microb Pathog. 2008 Jan 17;44(6):512–523. doi: 10.1016/j.micpath.2008.01.003

Figure 5.

Figure 5

LpnA stimulates secretion of CXCL8 and CCL2 by huMDM. HuMDM were treated with medium alone (Unstim), recombinant LpnA ranging from 0.01 µg/ml to 1.0 µg/ml, or 0.01 µg/ml of E. coli LPS for 24 h at 37°C. All samples of LpnA caused significant increases in the production of CXCL8 (A) and CCL2 (B) relative to that of unstimulated macrophages as measured by ELISA of cell-free conditioned media (P < 0.001). Bars represent the means ± SD of three replicate samples. Although the magnitude of the response differed from donor to donor, LpnA at concentrations of 0.01 µg/ml or above significantly increased secretion of these chemokines in two additional experiments.