Table 1.
Primer and TaqMan™ probes
| Primer and Probe Description | Primer sequences for PCR (forward (F) and reverse (R)) Probe sequences for TaqMan™ analysis |
| Hypoxanthin-guanine-phosphoribosyl-transferase | F: 5'-TGGAAAGAATGTCTTGATTGTTGAAG-3' |
| R: 5'-ATCTTTGGATTATGCTGCTTGACC-3' | |
| Probe: 5'(VIC)-ACACTGGCAAAAVAATGCAAACCTTGCT-(TAMRA)3' | |
| β-Actin | F: 5'-TCATCACCATCGGCAACG-3' |
| R: 5'-TTCCTGATGTCCACGTCGC-3' | |
| Probe: 5'(VIC)-CCTTCCTGGGCATGGAGTCCTGC-(TAMRA)3' | |
| Interleukin 1 beta (IL-1β) | F: 5'-GGTTTCTGAAGCAGCCATGG-3' |
| R: 5'-GATTTGCAGCTGGATGCTCC-3' | |
| Probe: 5'(FAM)-AAAGAGATGAAGTGCTGCACCCAAAACCTG-(TAMRA)3' | |
| Interleukin 6 (IL-6) | F: 5'-GGGTAGGGAAGGCAGTAGCC-3' |
| R: 5'-GAATCCCTCTCCACAAGCG-3' | |
| Probe: 5'(FAM)-CTTCAGTGGAGTCGCCTTCTCCCTAA-(TAMRA)3' | |
| Interleukin 8 (IL-8) | F: 5'-TTCTGCAGCTCTCTGTGAGGC-3' |
| R: 5'-GGTGGAAAGGTGTGGAAGTC-3' | |
| Probe: 5'(FAM)-TTCTGGCAAGAGTAAGTGCAGAACTTCGATG-(TAMRA)3' | |
| Interleukin 10 (IL-10) | F: 5'-TTGGAGCTTGCTAAAGGCACT-3' |
| R: 5'-CGGCGCTGTCATCAATTTCT-3' | |
| Probe: 5'(FAM)-CACCTCCTCCACGGCCTTGCTCTT-(TAMRA)3' | |
| Surfactant protein B (SP-B) | F: 5'-TCC GCT GGT CGT TGA TCA C-3' |
| R: 5'-GTT TGC ACA GGC CCA AGT G-3' | |
| Probe: 5'(FAM)-CAG AGC CAA ATG AAC CTG AAG GCC ATC-(TAMRA)3' | |
| Surfactant protein C (SP-C) | F: 5'-CAC CTT CTC CAT TGG CTC TAG TG-3' |
| R: 5'-ATA CTC TGC GGA GAC ATC TTC ATG-3' | |
| Probe: 5'(FAM)-TGA CTA CCA GCG GCT CCT GAT TGC C-(TAMRA)3' |