Table 1.

PCR Primers used throughout this work

Purpose	Target region	Primer name	Sequence (5' → 3')
Determination of the are of deletion	ORF 1	rgx1startfw	TAAGTTTAGCTAAGGCAGAG
		rgx1startrv	AAATTAAAGAGGCTAGGCTG
	ORF 3	rgx1orf3fw	ACTCGTATGCTTGACTTTCTG
		rgx1orf3rv	CTATCTTGTTTAACCCAGTCAC
	ORF 4	rgx1orf4fw	CTCTTTACTCAATCGCCGAC
		rgx1orf4rv	CCAACAGCAGATTACGAGAC
	ORF 5	rgx1orf5fw	CTTATCCATTTCCGTGTTCC
		rgx1orf5rv	CTAGAATTCAAAGTCGCCAG
	ORF 10	rgx1orf10fw	TATAAGTCTGTTTGGTCCCTG
		rgx1orf10rv	GTATTACTCACGCTTTACCTG
	ORF 14	rgx1orf14fw	TAATAACCCAACCTCTACAC
		rgx1orf14rv	ACACGAGCAGAATATTAGTC
	ORF 15	rgx1orf15fw	GTACTCTAGAGACAGAATGGTGGCGCTATCGTC
		rgx1orf15rv	GTCAGGATCCAGAGCGGTATCAAGCAGTATCC
	ORF 16	rgx1orf16fw	ATGTCTACCTTACTGGATACTG
		rgx1orf16rv	CCGTCACATATTACAAGTTCTG
	ORF 20	rgx1orf20fw	ATCCACCTCATCGTTATTCC
		rgx1orf20rv	GTGGTTAAGAACAATGGAGC
	ORF 26	rgx1orf26fw	GTTGACACCATCTACTGCTG
		rgx1orf26rv	GCTTATCTACGCCGATTCTG
	ORF 28	rgx1orf28fw	GTGTTTAACCATAGCCAGAC
		rgx1orf28rv	TCTAGGTAAGCCTTCAAGAG
	ORF 29	rgx1orf29fw	GAACTCCCTAACTTCATCTCAG
		rgx1orf29rv	CAACCATCTCACTAGACCAC
	ORF 31	rgx1orf31fw	TTCTTGTCAACCCAACAGTC
		rgx1orf31rv	TTTCTACCACCTTTGAGCAG
	ORF 34	rgx1orf34fw	GATACGGTAGATATTCTTCC
		rgx1orf34rv	GAGAGTACATTCTAACTACC
Determination of the downstream end of the deletion	+500	orf31do05kFw	GAGGTACAGCGAATACAC
	+1000	orf31do1kFw	CAGATGGTGTTCAAGTTCTC
	+1500	orf31do15kFw	CTCTTGCTTCCATCAAATCAG
	+2000	orf31do2kFw	CGTCAAGTGTTATGTTGTCC
	+2500	orf31do25kFw	CGAGATGAAAGATTCACAGC
	+3000	orf31do3kFw	GAGGTATCGTGTTCAATGTC
Genome Walking		GWqm9414gsp1	CCTTATCACTACCTTCCACCTCCATCTTATACCC
		GWqm9414gsp2	CCTCCATCTTATACCCTCTACCCAATTCCC
		GWRUTC30gsp1	TACCGCCATCGCAGACTGTTCCCTTTC
		GWRUTC30gsp2	TCACTATGAGACGGCAG
Cre1 amplification		Cre1fw	TCTCTGGGCTCTCTTGTAACC
		CreIIr	TGCCACTCCTCCTCATGTCAT
		creF	GTACTTTGGCCCTCGCTGAG
		creR	CCAGACTGCATAAGGATTCCC
		creRUTr	AGCAATCAGGTGCAGATATCAC