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. 2008 Aug 1;4(8):e1000143. doi: 10.1371/journal.pgen.1000143

Figure 7. Cytoplasmically localized FHY1 CT induces a dominant negative phenotype.

Figure 7

(A) Morphology of seedlings expressing FHY1 CT. Wild-type (Ler), fhy1-1, and phyA-201 seedlings as well as transgenic lines expressing different FHY1 167–202 ( = FHY1 CT) constructs in wild-type background were grown for 5 days in the dark or in FR (15 µmol m−2 s−1). #2590, #2619, #2643; Ler P35S∶YFP-FHY1 167–202 (Ler YFP-FHY1 CT) #4520, #4527; Ler P35S∶NLS-YFP-FHY1 167–202 (Ler NLS-YFP-FHY1 CT, i.e. artificial FHY1) #4578, #4597; Ler P35S∶NES-YFP-FHY1 167–202 (Ler NES-YFP-FHY1 CT) (B) Cytoplasmically localized FHY1 CT inhibits phyA nuclear accumulation. Ler P35S∶NES-YFP-FHY1 167–202 was crossed into phyA-201 PPHYA∶PHYA-CFP. F1 seedlings were grown for 3 days in the dark, irradiated for 6 h with FR (15 µmol m−2 s−1) and used for microscopic analysis. The scale bars represent 10 µm.