Figure 1.

A representative Lineweaver-Burk plot of minocycline transport by SCC-25 cells at 37° C. Left inset: Temperature-dependence of minocycline accumulation by SCC cells. Cell monolayers were incubated in HBSS at 37° and 2° C. Minocycline (10 μg/ml) was added and uptake was monitored over the indicated time intervals. Data are representative of three experiments. Right inset: A representative plot of ciprofloxacin transport at 37° C.