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Efflux of ciprofloxacin and minocycline from loaded SCC cells. Cells were loaded to a steady state concentration by incubation with HBSS containing 35 μg/ml ciprofloxacin or minocycline for 15 minutes at 37° C. These solutions were rapidly aspirated and replaced with 37° C HBSS. The decrease in intracellular antimicrobial content was monitored over 25 minutes.
