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. 2008 Aug;132(2):209–222. doi: 10.1085/jgp.200809978

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© 2008 Vaid et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 11. — Immobilization of the selectivity filter gate abolishes the fluorescence dequenching. (A and B) Representative fluorescence signals recorded from Kv1.5 A397C R487V mutant channels during a 100-ms voltage clamp pulse from −80 to +60 mV. Similar recordings were obtained from four (R487V) or nine (W472F) other cells. (C and D) Fluorescence signals recorded from Kv1.5 A397C H463G mutant channels during a 100-ms voltage clamp pulse from −80 to +60 mV. Similar recordings were obtained from four (R487V) or five (H463G) other cells.