Figure 10.

Effects of Ts3 in the fluorescence changes that track the movement of the S4 segment of domains II and III, using the mutant channel S660C and L1115C stained with TMRM. Traces were obtained as described in Fig. 7. (A) F-V curves obtained from S660C channels before (white symbols) and after (black symbols) the treatment with 200 nM Ts3 (mean ± SEM, n = 3). The fluorescence recorded at each potential was normalized to the maximal fluorescence. Solid lines are the curves obtained by fitting the data with the function 3. (B) F-V curves obtained from L1115C channels before (white symbols) and after (black symbols) the treatment with 200 nM Ts3 (mean ± SEM, n = 3). The fluorescence recorded at each potential was normalized to the maximal fluorescence. Solid lines are the curves obtained by fitting the data with function 3.These experiments were performed at room temperature.