Figure 2. Sequencing results demonstrating loss of the wild-type EPHB2 allele in the G787R carrier. EPHB2 gene sequencing results of genomic DNA extracted from paired tumor and adjacent normal colon mucosa are shown.

Relative to the intensities of the guanine bands in the sequencing reaction, the intensity of the guanine nucleotide at position 2359 is substantially reduced in the tumor sample, suggesting loss of the wild type allele (G) in the tumor but not in the adjacent normal tissue (arrow shows nt. 2359).