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. 2008 Aug 6;3(8):e2890. doi: 10.1371/journal.pone.0002890

Figure 3. Increased insulin sensitivity in Fsp27 −/ and ob/ob/Fsp27 −/− mice.

Figure 3

GTT (A) and ITT (B) experiments using 10-week-old wild type (WT), Fsp27 mutant (Fsp27 −/−), leptin deficient (ob/ob) and leptin/Fsp27 double deficient (ob/ob/Fsp27−/−) mice. 6 mice from each group were used for the experiment. *p<0.05; **p<0.01; ***p<0.001. Western blot analysis for levels of IRS-1 and insulin stimulated IRS-1 tyrosine phosphorylation (p-IRS) in the WAT of wild type (WT) and Fsp27 mutant (Fsp27−/−) mice (C) and in the WAT of leptin deficient (ob/ob) and leptin/Fsp27 double deficient (ob/ob/Fsp27−/−) mice (D). . E & F. Western blot analysis of total AKT2, phosphor-AKT2 (pAKT2) and GLUT4 protein levels in WAT of wild type (WT), Fsp27 mutant (Fsp27−/−), leptin deficient (ob/ob) and leptin/Fsp27 double deficient (ob/ob/Fsp27−/−) mice. β-actin was used as loading control.