Figure 4.

Expression Profiling of DRIP1 in Different Tissues, and the Degradation of DREB2A-GFP Protein Is Inhibited by the MG132 Proteasome Inhibitor.

(A) GUS staining of DRIP1pro:GUS transgenic plants from different growth stages. (a) Gene expression from mature seeds. (b) Ten-day-old seedlings growing on a GM agar plate. (c) Aerial portions of 3-week-old seedlings. (d) Higher magnification image of (c) for observing cotyledons. (e) Root tips and elongation zones from 3-week-old seedlings. (f) Flowers from 7-week-old plants. (g) Siliques from 7-week-old plants. Bars in each panel indicate actual lengths.

(B) A native DREB2A promoter sequence was constructed to drive GFP-DREB2A gene expression. Three-week-old plants treated (or mock-treated) with MG132 under dim light overnight were observed with fluorescence microscopy.