Table 2.
Glycosyl Residue Linkage Analysis of Fractionated Cell Walls of 7-d-Old Etiolated Seedlings
| Hot Ammonium Oxalate Extraction
|
4 n KOH Extraction
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Fractions Residuea | Col-0 | xxt1 | xxt2 | xxt1 xxt2 | Col-0 | xxt1 | xxt2 | xxt1 xxt2 |
| T-Fucp | 1.7 | 1.9 | 1.6 | 1.8 | 0.9 | 1.3 | 1.0 | 0.5 |
| 2-Rhap | 5.3 | 5.4 | 5.5 | 13.6 | ND | ND | ND | ND |
| 2,4-Rhap | 4.0 | 4.6 | 4.6 | 1.0 | ND | ND | ND | ND |
| T-Araf | 7.6 | 6.0 | 6.5 | 8.9 | 2.3 | 3.2 | 3.5 | 5.0 |
| 2-Arap | ND | ND | ND | ND | 1.8 | 3.0 | 4.3 | 1.9 |
| 3-Araf | 7.5 | 4.8 | 5.0 | 5.6 | 1.8 | 1.4 | 1.7 | 0.0 |
| 5-Araf | 18.8 | 11.0 | 14.4 | 17.7 | 3.4 | 3.2 | 3.9 | 6.6 |
| T-Xylp | 1.9 | 2.0 | 1.9 | 1.7 | 2.0 | 2.7 | 2.0 | 1.3 |
| 2-Xylpb | 1.0 | 1.3 | 1.2 | 0.6 | 1.8 | 2.1 | 1.8 | 1.5 |
| 4-Xylpb | 5.3 | 8.5 | 6.5 | 7.6 | 9.0 | 13.5 | 13.0 | 30.2 |
| 2,4-Xylp | 4.3 | 4.0 | 6.3 | 3.4 | 2.3 | 3.3 | 3.8 | 2.9 |
| T-Galp | 7.3 | 9.7 | 10.0 | 12.9 | 1.8 | 2.2 | 2.0 | 3.8 |
| 2-Galp | ND | ND | ND | ND | 3.3 | 3.6 | 2.9 | 1.9 |
| 4-Galp | 8.5 | 6.9 | 6.9 | 10.0 | 3.7 | 1.8 | 1.8 | 2.4 |
| T-Manp | ND | ND | ND | ND | 1.1 | 1.6 | 2.4 | 4.2 |
| 4-Manp | ND | ND | ND | ND | 3.8 | 4.2 | 4.7 | 8.1 |
| 4,6-Manp | ND | ND | ND | ND | 4.7 | 2.7 | 2.8 | 5.1 |
| T-Glcp | 2.8 | 3.1 | 3.1 | 2.6 | 1.0 | 0.1 | 1.6 | 3.1 |
| 3-Glcp | ND | ND | ND | ND | 5.2 | 5.6 | 7.8 | 5.4 |
| 4-Glcp | 10.1 | 13.3 | 11.2 | 4.3 | 19.3 | 13.7 | 13.1 | 12.6 |
| 6-Glcp | 4.5 | 4.0 | 3.8 | 4.2 | 1.6 | 1.2 | 1.8 | 1.3 |
| 4,6-Glcp | 9.6 | 13.2 | 11.4 | 4.0 | 29.1 | 29.5 | 24.1 | 2.2 |
a
Glycosyl residues are expressed as percentage of total peak areas.
b
The peak area values for 2-Xyl and 4-Xyl were calculated by multiplying the relative percentage of m/z 190 and m/z 189, respectively, by the total ion count of the peak.
ND, not detected.