Table 3.
Analysis of Driselase-Digested AIR Preparations of 7-d-Old Etiolated Seedlings
| Monosaccharide (μg mg−1 AIR) Composition of Driselase-Resistant Materialb
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Genotype | IPa (μg mg−1 AIR) | Rhamnose | Fucose | Arabinose | Xylose | Mannose | Galactose | Glucose |
| Col-0 | 14.13 ± 0.07 | 3.38 ± 0.33 | 0.21 ± 0.05 | 7.29 ± 0.35 | 4.76 ± 0.36 | 1.32 ± 0.14 | 8.17 ± 0.71 | 21.23 ± 2.57 |
| xxt1 | 12.69 ± 0.15* | 3.43 ± 1.63 | 0.09 ± 0.10 | 6.58 ± 2.13 | 4.01 ± 1.27 | 1.33 ± 0.66 | 7.73 ± 2.78 | 19.00 ± 6.60 |
| xxt2 | 11.19 ± 0.10* | 3.47 ± 0.74 | 0.04 ± 0.05* | 6.15 ± 2.11 | 3.92 ± 1.30 | 1.42 ± 0.15 | 6.31 ± 1.88 | 14.82 ± 6.89 |
| xxt1 xxt2 | ND* | 2.78 ± 0.11 | ND* | 6.48 ± 0.33 | 3.91 ± 0.23 | 1.29 ± 0.09 | 4.73 ± 0.16* | 15.88 ± 1.45 |
a
HPAEC analysis of Driselase-susceptible AIRs.
b
GC-MS analysis of monosaccharide alditol acetate derivatives released by Saeman hydrolysis of Driselase-resistant AIR.
Values are derived from n = 3 biological repetitions ± sd. *Statistically significant difference with respect to the wild type (Col-0; P < 0.025). Trace indicates trace amount detected but not quantified. ND, not detected.