Figure 7.

Requirement of Both WIP and WIT Family Members for RanGAP NE Targeting in Root Tip Cells.

(A) In the wild type, a complex containing both a WIP and a WIT family member is present at the NE and RanGAP is bound to this complex.

(B) In wit1-1 wit2-1, WIP1 abundance and NE association are unchanged. However, in the absence of WIT family members, only a small amount of RanGAP is associated with the NE.

(C) In wip1-1 wip2-1 wip3-1, WIT1 abundance is drastically reduced. Remaining WIT1 is likely associated at least in part with the NE but is not sufficient for efficient RanGAP targeting.

(D) Overexpressing GFP-WIT1 in wip1-1 wip2-1 wip3-1 does not alter RanGAP localization, indicating that abundant GFP-WIT1 at the NE (which complements wit1-1 wit2-1) is not sufficient for RanGAP targeting in the absence of WIP family members.

The size of the symbols in (A) to (D) represents the abundance of the molecule in the respective cellular location.