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Ubiquitination of SPIN1 by SPL11 and TAP-SPIN1 Accumulation in SD and LD.
SPL11 monoubiquitinates SPIN1 in vitro. The arrowhead indicates the position of monoubiquitinated SPIN1; the arrow indicates unubiquitinated SPIN1. The unrelated E3 ligase Os SINAT5 was used as a negative control (At UBC7 was used as the E2 enzyme in the Os SINAT5 reaction, while At UBC9 was used in the SPL11 reaction).
