Figure 8.

Overexpression of Spin1 Causes Delayed Flowering under Both SD and LD Conditions.

(A) Protein gel blot detection of the accumulation of TAP-SPIN1 protein in a T4 line overexpressing Spin1-TAP. Line 32-3-5 is segregating for flowering time in SD.

(B) Days to heading under SD and LD in T4 RNAi and overexpressing Spin1 (Spin1-TAP) lines and on the untransformed recipient cultivar NPB. + and – indicate siblings from the same T3 family that do and do not contain the RNAi or overexpressing construct. The asterisk indicates a plant that did not flower after 230 d.

(C) RT-PCR/DNA gel blot analysis of Hd1, Hd3a, SOC1, and Ubq in Spin1-TAP line 11-15 and NPB under SD and LD over a 24-h period in 55-d-old plants.

(D) to (G) Quantification of gene expression relative to Ubq of the Hd1 and Hd3a blots shown in (C). White and black rectangles represent light and dark conditions over a 24-h period.

Error bars indicate sd from three independent experiments.