Figure 3.

WUS Transcripts Are Detected in the Outermost Cell Layers, Instead of the Usual OC, in bard1-3 SAMs.

(A) and (B) In situ hybridization of BARD1 and WUS, respectively, in wild-type Arabidopsis.

(C) and (D) In situ hybridization of BARD1 and WUS, respectively, in bard1-3. Bars = 20 μm.

(E) QRT-PCR analysis of WUS mRNA levels in the wild type and the bard1-3 mutant. Mean (±se) comparative threshold (CT) values were calculated from triplicate QRT-PCR experiments using independent RNA samples prepared from different batches of Arabidopsis plants.