Figure 10.

Both the N and C Termini of phyA and phyB Are Necessary for Interaction with PIF1.

(A) and (C) Schematic representations of the GAD-PIF1 bait (left) and various phy preys (right) used for coimmunoprecipitation assays.

(B) and (D) Autoradiographs showing interactions of full-length PIF1 with the Pr and Pfr forms of full-length and truncated versions of phyA (B) and phyB (D). Input and pellet fractions are shown from the in vitro coimmunoprecipitation assays performed as described (see Figure 5) (Huq et al., 2004). The CT samples are from the C-terminal halves of either phyA or phyB that do not bind to chromophore and are not labeled as Pr or Pfr.