Figure 2.

phyA Plays a Dominant Role during the Initial Light Exposure, While phyB, phyD, and Other phys Regulate PIF1 Stability under Prolonged Light Exposure.

Protein gel blots showing native PIF1 levels in wild-type, phyA, phyB, and phyAB backgrounds. Four-day-old dark-grown seedlings were exposed to FRp (A), Rp (B), or continuous red light (Rc) ([C] and [D]) at the indicated fluences and then incubated in the dark for the times indicated (except Rc) before harvesting for protein extraction. phyAB and phyABD shown in (D) are in the Landsberg erecta (Ler) ecotype; all other mutants are in the Columbia ecotype.