Figure 2.

Effects of dimemorfan on increased intracellular calcium concentration, induced by different activators, in human neutrophils. Changes of intracellular calcium concentration in fura-2 AM preloaded human neutrophils (2 × 10⁷ cells mL⁻¹) pre-treated with 10 or 20 μM dimemorfan (+D10 or +D20) were measured after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP, 1 μM), AlF₄⁻ (10 mM NaF plus 30 μM AlCl₃), or thapsigargin (10 μM). Net increase of intracellular calcium concentration (nM) (Δ[Ca²⁺]_i) was calculated by subtraction of the value of [Ca²⁺]_i in resting cells (82±3.0 nM) from each data point. Data are expressed as the mean±s.e.m. (n=3–5 for each group). ^*P<0.05, compared with the corresponding activator only group, ^†P<0.05, compared with AlF₄⁻+D10 or AlF₄⁻+D20, respectively, by one-way ANOVA followed by the Student-Newman-Keuls t-test.