Table 2.

Effects of dimemorfan on NOX activity from BV2 cells and human neutrophils

Treatment	Maximum inhibition (%) in BV2 cells	IC50 (μM) in neutrophils
Dimemorfan	44±2*	47±3*
Diphenylene iodonium	90±5	24±5

Abbreviation: NOX, NADPH oxidase.

Cell lysate from BV2 cells and human neutrophils was co-incubated with 10–50 μM of dimemorfan in the presence of lucigenin (50 μM) and NADPH (200 μM). NOX activity was determined from the chemiluminescence. Data are calculated as the IC₅₀ or the percent of maximum inhibition at 50 μM when IC₅₀ value is not obtainable, and expressed as the mean±s.e.mean of five to six experiments performed on different days using cells from different passages (BV2) or healthy donors (neutrophils). ^*P<0.05, compared with diphenylene iodonium group, by t-test.