Table 1.
Effect of OncoA on GP Ibα (CD 42b), P-selectin (CD62P), GP IIb (CD41a) and GP Ia (CD49b) and GPIIa (CD29)
| Monoclonal antibody |
Fluorescence intensity |
||||
|---|---|---|---|---|---|
| Control |
OncoA (μM) |
||||
| 41 | 82 | 164 | 328 | ||
| Anti-CD42b | 193.8±19.8 | 167.0±13.6# | 125.9±12.5* | 104.2±2.1* | 107.0±1.7* |
| Anti-CD41a | 340.4±26.5 | 333.7±26.3 | 349.8±31.1 | 338.5±24.0 | 337.4±30.0 |
| Anti-CD62P | 17.2±1.3 | 17.6±1.1 | 18.1±0.1 | 20.4±1.6 | 18.0±1.5 |
| Anti-CD49b | 31.6±0.8 | 31.6±0.8 | 30.8±0.8 | 29.8±0.2 | 30.0±1.5 |
| Anti-CD29 | 211.4±9.6 | 215.2±3.7 | 216.7±2.76 | 222.9±6.0 | 217.8±6.9 |
Samples of PRP (3 μL) were incubated at 37 °C, for 5 min, with vehicle or OncoA. Then, 40 μL of 10% bovine fetal serum in phosphate-buffered saline solution, pH 7.4 was added with the specific antibody. After 30 min incubation at room temperature, the reaction was terminated by 1% paraformaldehyde (300μL) and the samples analysed by flow cytometry. Results are the mean±s.e.m. of four experiments.
#P<0.05 vs control; *P<0.001 vs control, ANOVA for repeated measures followed by Tukey–Kramer for multiple comparisons.