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. 2008 May 28;10(3):R60. doi: 10.1186/ar2431

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Copyright © 2008 Reisch et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoprecipitation of DREAM protein from different tissues (arrows). The DREAM antibody recognizes the glutathione S-transferase-tagged recombinant protein (10 ng; predicted size of 54 kDa) and the native protein from neuronal tissue (human occipital cortex). The immunoprecipitations show the two antibody bands detected by the secondary goat anti-mouse antibody (heavy and light chains), and in the last lane, resembling the immunoprecipitation from neuronal tissue, a DREAM-specific signal of the expected size (~30 kDa) was detectable. DREAM, downstream regulatory element antagonist modulator; IP, immunoprecipitation; PBMC, peripheral blood mononuclear cell; SFLC, synovial fibroblast-like cell.