Table 1.

Sequences of oligonucleotides used in polymerase chain reaction (PCR) and real-time PCR as well as for the generation of small interfering RNAs

Primers for conventional DREAM PCR
	Forward	Reverse
DREAM	5'-CCGGCTAAGGAAGTGACAAA-3'	5'-CAAAGGCGTTGAAGAGGAAG-3'
nDREAM	5'-GAAGGAGGGTATCAAGTG-3'	5'-TAAATGAGTTTGAAGGTGTC-3'
Primers for SYBR green assay real-time PCR
	Forward	Reverse
c-fos	5'-TAAATGAGTTTGAAGGTGTC-3'	5'-ACAGGAACCCTCTAGGGAAGA-3'
Oligonucleotides for the synthesis of siRNAs
	Sense	Antisense
siRNA1	5'-AAGGACAGGATCCACTTGACCTATAGTGAGTCGTATTA-3'	5'AAGGTCAAGTGGATCCTGTCCTATAGTGAGTCGTATTA3'
siRNA2	5'-AAGGTGAACTTGGTCTGGGCCTATAGTGAGTCGTATTA3'	5'-AAGGCCCAGACCAAGTTCACCTATAGTGAGTCGTATTA-3'
siRNA3	5'-AAGTAGAGATTAAAGGCCCACTATAGTGAGTCGTATTA-3'	5'-AAGTGGGCCTTTAATCTCTACTATAGTGAGTCGTATTA-3'
siRNA4	5'-AAGCTCATGATGTTCTCATCCTATAGTGAGTCGTATTA-3'	5'-AAGGATGAGAACATCATGAGCTATAGTGAGTCGTATTA-3'
siRNA5	5'-AAGTGTAGCAATCTGTTCACTATAGTGAGTCGTATTA-3'	5'-AAGTGAACAGATTGCTACACTATAGTGAGTCGTATTA-3'
siRNA-GFP	5'-ATGAACTTCAGGGTCAGCTTGCTATAGTGAGTCGTATTA-3'	5'-CGGCAAGCTGACCCTGAAGTTCTATAGTGAGTCGTATTA-3'
T7	5'-TAATACGACTCACTATAG-3'

siRNA3 (binding in the coding region of exon 6) and siRNA4 (spanning the non-coding exons 8 and 9) were used to interfere with endogenous DREAM mRNA and to analyze downstream target genes of DREAM gene regulation like pdyn and c-fos. Two different primers for DREAM are given. DREAM, downstream regulatory element antagonist modulator; GFP, green fluorescence protein; siRNA, small interfering RNA.