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(a) Anomalous difference electron-density map (red) contoured at 4σ computed for a gold derivative using the phases obtained from ab initio molecular replacement. 2F o − F c map contoured at 1σ computed from ab initio phases with omitted residues shown in green for (b) the complete transmembrane ensemble, (c) for a single transmembrane helix and (d) for the cytoplasmic domain (for clarity, the electron density is shown for only one cytoplasmic helix).
