Figure 4.
DNA damage progresses in parallel with the kinetics of up-regulation of MICA and MICB in Dicer knockdown cells. Cells were transfected with Dicer siRNAs. (A) γ-H2AX (▪) and RPA70 (•) staining was performed at the indicated time points. (B) Levels of MICA (▪), MICB (•), and Dicer (broken lines) transcripts were measured by real time RT-PCR. Means ± SD of three independent experiments are shown.