Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Jul 28;182(2):301–313. doi: 10.1083/jcb.200712036

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Cao et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Nuf is required for proper RhoGEF2 recruitment to the furrow. (A) Images of fixed WT, RhoGEF2, and nuf embryos are shown in cycle-13 metaphase and cellularization (staged by DNA, blue) at the sections crossing the furrow. (B) Compared with WT, RhoGEF2 is more diffuse at the furrow in nuf and Rab11 embryos at cycle-13 prophase. Green, F-actin; red, RhoGEF2. (C) Line plots of RhoGEF2 (red curves) and Dlg (green curves) signal intensities (normalized to the maximum signal of each plot into a 0–1 scale) crossing the furrows (red lines in B). WT exhibited a single focused RhoGEF2 peak corresponding to the Dlg peak. In nuf and Rab11 embryos, RhoGEF2 showed unfocused peaks that did not generally correspond with the Dlg peak. (D and E) Rho1 and Diaphanous (red) are not concentrated in furrow regions lacking F-actin (green) but localize properly at regions maintaining F-actin in nuf embryos. (F) At cycle-13 interphase, RhoGEF2 (red) is diffusely localized around the furrow, whereas Anillin (green) is localized normally (arrows). (G) Nuf shows dominant genetic interactions with components of the RhoGEF2–Rho1 pathway. Cycle-13 prometaphase or metaphase embryos (staged by DNA; blue) derived from females with different genetic background were stained with phalloidin to show the furrow structure. (RhoGEF2/Rho1), (RhoGEF2/+; nuf/+), (Rho1/+; nuf/+), (chic/+; nuf/+), and (dia/+; nuf/+) embryos display abnormal furrow morphologies (arrows indicate furrow breaks or weak furrows), whereas (Arpc1/+; nuf/+) embryos have relatively normal furrow morphology. Summary of phenotypic frequencies is shown in Table I. Bars: (A and G) 10 μm; (B–F) 5 μm.