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. 2008 Jul 28;182(2):315–325. doi: 10.1083/jcb.200710067

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© 2008 Niessen et al.

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Figure 6. — Induction of Snail by TGF-β2 is synergistically enhanced in Dll4-activated endothelial cells. (A) qRT-PCR for Snail mRNA in vector- or Dll4-activated HMEC treated with 2.5 ng/ml TGF-β2 for the indicated times (n = 3). *, P < 0.05. (B) Immunoblots for Snail and Slug in vector- or Dll4-activated HMEC treated with 2.5 ng/ml TGF-β2. (C) qRT-PCR for Snail, Slug, Hey1, and Smad7 mRNA in vector- or Dll4-activated HMEC treated with 10 μM DMSO or DAPT for 16 h followed by treatment with 2.5 ng/ml TGF-β2 for 3 h (n = 3). *, P < 0.05. (D) qRT-PCR for Snail, Slug, Hey1, and Smad7 mRNA in vector- or Dll4-activated HMEC treated with 20 or 50 ng/ml BMP2 (n = 3). Error bars show SEM.