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. 2008 Jul 28;182(2):315–325. doi: 10.1083/jcb.200710067

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© 2008 Niessen et al.

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Figure 7. — Increased Snail expression compensates for Slug deficiency. (A) qRT-PCR analysis for Snail and Slug of whole hearts isolated from E9.5, 10.5, and 11.5 wild-type (wt) and Slug^−/−embryos (n = 3). *, P < 0.05. Error bars show SEM. (B) In situ hybridization for Snail in E10.5 and 11.5 wild-type (wt) and Slug^−/− hearts (n = 3; five embryos in each replicate). Bars, 250 μm. (C) Quantitation of EMT in AV canal explants from E9.5 wild-type (wt) or Slug^+/− and Slug^−/− embryos treated with 5 ng/ml TGF-β2 or vehicle (UT). (D) Quantitation of EMT in AV canal explants from E10.5 wild-type (wt) or Slug^+/− and Slug^−/− embryos transduced with shRandom or shSnail constructs.