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. Author manuscript; available in PMC: 2008 Oct 31.

Published in final edited form as: J Am Chem Soc. 2007 Oct 5;129(43):12946–12947. doi: 10.1021/ja076222f

Partial ¹H NMR spectrum (500 MHz) of UMP from decarboxylation of OMP (2 mM) catalyzed by OMPDC from S. cerevisiae (24 nM) in 50/50 (v/v) H₂O/D₂O at pL 7.3 and 25 °C. Key: (▼) Doublet due to the C-6 proton of [6-¹H]-UMP; (•) Doublets (not resolved) due to the anomeric protons of [6-¹H]-UMP and [6-²H]-UMP; (×) Doublet due to the C-5 proton of [6-¹H]-UMP; Singlet due to the C-5 proton of [6-²H]-UMP.

Inline graphic — Partial ¹H NMR spectrum (500 MHz) of UMP from decarboxylation of OMP (2 mM) catalyzed by OMPDC from S. cerevisiae (24 nM) in 50/50 (v/v) H₂O/D₂O at pL 7.3 and 25 °C. Key: (▼) Doublet due to the C-6 proton of [6-¹H]-UMP; (•) Doublets (not resolved) due to the anomeric protons of [6-¹H]-UMP and [6-²H]-UMP; (×) Doublet due to the C-5 proton of [6-¹H]-UMP; Singlet due to the C-5 proton of [6-²H]-UMP.