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. 2008 Jul 24;118(8):2771–2784. doi: 10.1172/JCI32709

Figure 3. Effects of poly(I:C) on cytokines and IFNs.

Figure 3

Mice were exposed to CS or RA for 2 weeks and then randomized to receive poly(I:C) (50 μg) or vehicle control. The levels of BAL IL-18, IFN-α/β, IL-12/IL-23 p40, and IFN-γ in vehicle-treated mice breathing RA or CS were near or below the limits of detection of these assays. The effects of poly(I:C) in mice exposed to CS (filled circles) or RA (open circles) are illustrated (A–D). The localization of IL-18 was accomplished using IHC (E). In the lungs from mice exposed to CS plus poly(I:C), selected IL-18–containing macrophages are highlighted with arrows, and a high-power image can be seen in the insert. The values in parts A–D represent the mean ± SEM of evaluations in a minimum of 5 mice. Part E is representative of 3 similar experiments. *P < 0.05; ***P < 0.001. Original magnification, ×20 (E); ×100 (insert).