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. Author manuscript; available in PMC: 2008 Jul 24.
Published in final edited form as: J Biomed Opt. 2008;13(3):031203. doi: 10.1117/1.2939094

Fig. 5.

Fig. 5

The measurement of the changes in the donor signals from either the (a) mTFP-5aa-Venus or (b) mTFP-TRAF-Venus fusion proteins after acceptor photobleaching. Spectral measurements were acquired from cells expressing the indicated fusion proteins; the calibration bar indicates 10 μm. The linked Venus fluorophore was then photobleached by more than 70% using the 514-nm laser line. The spectral measurements were then reacquired under identical conditions to the first, and changes in the donor signal were measured. The dashed line in (a) indicates the change in the donor signal for the mTFP-5aa-Venus fusion protein. In contrast, there was little change in the donor signal for (b) mTFP-TRAF-Venus following acceptor photobleaching.