Table 1.
The characteristics of the Aequorea-based Cerulean and Venus, and the Clavularia-based mTFP.
FP | Ex (nm) | Em (nm) | Quantum yield | Intrinsic brightness | R0 with Venus |
---|---|---|---|---|---|
Cerulean | 433 | 475 | 0.62 | 27 | 54 Å |
mTFP | 462 | 492 | 0.85 | 54 | 61 Å |
Venus | 515 | 528 | 0.57 | 53 | NA |
Intrinsic brightness is the product of quantum yield × extinction coefficient (see Ref. 5). R0 with Venus is determined by R0 = 0.211 [(k2)(n−4)(QYD)| (Jλ)]0.1667. NA is not applicable.