. Author manuscript; available in PMC: 2008 Jul 24.

Published in final edited form as: J Biomed Opt. 2008;13(3):031203. doi: 10.1117/1.2939094

Table 2.

FRET measurements of Cerulean or mTFP fusions to Venus. Values for Cer-5aa-Venus for ps-FRET is ±SD, n=5, and for τ_DA (ns) is determined by E= 1 — (τ_DA/τ_D); see text.

Fusion protein	ps-FRET	Fluorescence lifetime
		τ_DA (ns)	E_FRET
Cer-5aa-Venus	49.5 ± 2	1.21 ± 0.12	51
Cer-TRAF-Venus	10.7 ± 3	2.32 ± 0.04	7
mTFP-5aa-Venus	55.3 ± 2	1.11 ± 0.06	55
mTFP-TRAF-Venus	11.5 ± 2	2.22 ± 0.07	11